What it's good for
- Generating multi-color 3D fluorescent images of high resolution
- 4 fluorescent colors plus a high contrast black and white DIC channel
- Fixed cells or sections on a slide with a coverslip (#1.5)
- Live cells in a coverslip bottom chamber or dish (#1.5)
- Cells in culture can be kept at 37 degrees with 5% CO2
- Multi-point time-lapse and panel acquisition
What it's NOT good for
- Thick tissue (>50 microns)
- Rapid timelapse
- Large travel distance
Prnciples of operation
Fluorescent light from a mercury lamp is filtered for excitation and emission. System eliminates out of focus light by calculations AFTER acquisition, producing sharper, brighter images.
Technical Information
- Microscope Base: Olympus IX70
- Incubation with CO2 (Solent)
- 4 fluorescent color channels and DIC:
- DAPI
- FITC
- Texas Red
- Cy-5
- Motorized X-Y-Z-T capability for live cell imaging
- Microscope objectives
- 10x (.30 NA)
- 20x (.70 NA)
- 40x (1.30 NA)
- 60x (1.40 NA)
- 100x (1.40 NA)