Zeiss LSM 880 Confocal with FAST Airyscan

What it’s good for

  • High resolution imaging at 1.7x resolution increase over standard confocal imaging
  • High acquisition speed, at 4x faster acquisition than confocal mode
  • High sensitivity with use of special detectors
  • Shuttle and Find capability to merge light and electron microscopy techniques for Correlative Microscopy
  • Acquisition of up to 3 color channels simultaneously
  • Acquisition of multiple channels sequentially
  • Acquisition of a high contrast DIC image
  • Fixed cells or tissues on a slide with a coverslip (#1.5)
  • Live cells, small organisms or tissues in a chamber or dish with a coverslip bottom (#1.5)
  • Spectral Deconvolution to correct for overlapping signals
  • Calcium Dynamics
  • FRET
  • Stimulus and bleaching applications

What it’s not good for

  • Live animal imaging (large animals such as mouse)

Principles of operation

Laser light of specific wavelengths is scanned across the sample and filtered before detection to produce a high resolution image composed of a small optical slice of the sample. Alternative detection at high resolution and sensitivity avoids discrimination of light with a pinhole, and utliizes a 32 detector array that can generate higher resolution images by detection of specific airy units.

Technical Information

  • Microscope: Ziess Observer inverted stand
  • Incubated with CO2
  • DIC channel
  • laser lines
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  • 488
  • 515
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  • 633