Zeiss Z.1 Light Sheet

What it’s good for

  • Thick specimens and whole organisms: Cleared tissue, zebrafish etc.
  • Fast acquisition speeds and low phototoxicity
  • Incubation for live imaging
  • Acquisition of up to 2 color channels simultaneously
  • Acquisition of multiple color channels sequentially

What it's not good for

  • Fixed cells or tissues on a slide with a coverslip (#1.5) or in a dish

Principles of operation

Laser light of specific wavelengths is passed through the sample as a sheet from 1 or 2 sides, perpendicular to the detection optics. Laser excitation, Emission filters and 2 CMOS cameras generate an image at a specific wavelength. Sample is moved through the sheet of light for Z stack acquisition. 

Technical Information

  • Filter based emission for all channels
  • 2 pico.edge CMOS cameras
  • 3 specimen chambers for different objectives and refractive index
  • Incubation for chamber
  • Laser Excitations:
  • 405nm
  • 445nm
  • 488nm
  • 514nm
  • 560nm
  • 638nm

Microscope objectives

  • EC Plan-Neofluar 5x (.16NA) Air
  • CLR Plan-Neofluar 20x Corr (1.0NA) dipping
  • Plan-Apochromatic 10x (.5NA) dipping
  • W Plan-Apochromatic 20x (1.0NA) dipping