What it's good for
- Live cells in glass coverslip bottom dish, chamber or well plate (#1.5)
- Fixed cells and thin tissues mounted on a slide with a coverslip (#1.5)
- High resolution imaging with little cell damage
- High speed live cell imaging (200 fps)
- High speed Z sectioning with Piezo Z-drive
- Multiple point time lapse
What it's not good for
- Point scanning confocal resolution
- Thick samples
Principles of operation
Spinning disk technology cuts laser light to decrease photoxicity and bleaching for long term live cell experiments, and creates a confocal effect for superior image quality.
Technical Information
- Microscope: Olympus IX81 inverted
- Yokogawa CSU-X1-A1 spinning disk head (5000rpms)
- EMCCD Hamamatsu 14 bit 1K x 1K camera
- Imaging speeds up to 200fps
- Incubated with CO2
- DIC and Phase Contrast
- 4 laser lines
- 405
- 488
- 560
- 640
- Microscope Objectives
- 10x (.30 NA) - 0.678um / pixel
- 20x ELWD (.45 NA) - 0.344um / pixel
- 40x (1.30 NA) - 0.169um / pixel
- 60x (1.42 NA) - 0.111um / pixel
- Piezo Z-drive for fast Z acquisition
- ASI XY stage
- Volocity software for acquisition and processing