What it’s good for
- Determining colocalization for multiple signals
- Acquisition of up to 3 color channels simultaneously
- Acquisition of up to 12 color channels sequentially
- Acquisition of a high contrast DIC image
- Fixed cells or tissues on a slide with a coverslip (#1.5)
- Live cells or tissues in a chamber or dish with a coverslip bottom (#1.5)
- Spectral Deconvolution to correct for overlapping signals
- Calcium Dynamics
- FRET
- Stimulus applications
- FRAP
- FLIP
- Photoactivation
- Photoconversion
- Uncaging
What it’s not good for
- Dim signal
- Thick tissues (>50 micons)
- Live Cells: Can be very harsh for some live cell applications
- Multipoint timelapse and panel acquisition
Principles of operation
Laser light of specific wavelengths is scanned across the sample and filtered before detection to produce a high resolution image composed of a small optical slice of the sample.
Technical Information
- Microscope: Olympus IX81 inverted
- Incubated with CO2
- Spectral grating system for 2 channels
- DIC channel
- 6 laser lines
- 405
- 458
- 488
- 515
- 543
- 633