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Olympus FV1000 Confocal with SIM Scanner

What it’s good for

  • Determining colocalization for multiple signals
  • Acquisition of up to 4 color channels simultaneously
  • Acquisition of up to 16 color channels sequentially
  • Acquisition of a high contrast DIC image
  • Fixed cells or tissues on a slide with a coverslip (#1.5)
  • Live cells or tissues in a chamber or dish with a coverslip bottom (#1.5) (only BSL1 Cell Lines)
  • Spectral Deconvolution to correct for overlapping signals
  • Calcium Dynamics
  • FRET
  • Stimulus applications with simultaneous activation and imaging
  • FRAP
  • FLIP
  • Photoactivation
  • Photoconversion
  • Uncaging

What it’s not good for

  • Dim signal
  • Thick tissues (>50 micons)
  • Live Cells: Can be very harsh for some live cell applications
  • Multipoint timelapse and panel acquisition

Principles of operation

Laser light of specific wavelengths is scanned across the sample and filtered before detection to produce a high resolution image composed of a small optical slice of the sample.

Technical Information

  • Microscope: Olympus IX81 inverted
  • Incubated with CO2
  • Spectral grating system for 2 channels
  • DIC channel
  • 6 laser lines
  • 405
  • 458
  • 488
  • 515
  • 543
  • 633
  • Microscope objectives
  • 10x (.40 NA)
  • 20x (.75 NA)
  • 40x (1.30 NA)
  • 60x (1.42 NA)

FluoView Viewer Software Download (serial number 8L62)

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