What it's good for
- Micro-dissection of tissues and isolation of live cell populations
- Special materials required
- Isolated material can be analyzed for DNA, RNA or Proteins
- Laser ablation and time lapse imaging
- Multipoint time lapse for live samples
What it's NOT good for
- Standard microscopic imaging
Principles of operation
UV laser is used to microdissect specific cells or areas out of sample. Microdissected material is collected with a sticky cap eppendorf tube. Careful control of power allows for precise ablation of small volumes.
Technical Information
- Microscope Base: Olympus IX81
- Incubation with CO2
- Solid state 355nm laser
- Color CCD camera
- High speed and quality monochrome Hamamatsu CCD camera
- 3 fluorescent color channels:
- DAPI
- FITC
- Texas Red
- DIC and Phase Contrast and color brightfield
- Motorized X-Y stage for multipoint live cell imaging
- Microscope objectives:
- 4x (.13 NA)
- 10x (.40 NA)
- 20x PH1 ELWD (.70 NA)
- 40x PH2 ELWD (.60 NA)
- 60x ELWD PSF Water (1.40 NA)
Consumable items