What it’s good for
- Determining colocalization for multiple signals
- Acquisition of up to 3 color channels simultaneously
- Acquisition of multiple color channels sequentially
- Acquisition of a transmitted light image
- Fixed cells or tissues on a slide with a coverslip (#1.5) or in a dish
- Live tissues or Live Animals
- Spectral Deconvolution to correct for overlapping signals
- Calcium Dynamics - Fast Scanning with Resonant Scanner
- FRET
- Stimulus applications
- FRAP
- FLIP
- Photoactivation
- Photoconversion
- Uncaging
What it’s not good for
- Live Cells: Can be very harsh for some live cell applications and an upright system is not ideal for live cells in a dish
Principles of operation
Laser light of specific wavelengths is scanned across the sample and filtered before detection to produce a high resolution image composed of a small optical slice of the sample.
Technical Information
- Microscope: Leica Upright
- Incubated with CO2
- Spectral for all channels
laser lines - 405
- 458
- 488
- 515
- 543
- 594
- 633
- Mai Tai HP 2-photon laser tunable from 720nm - 1040nm
Microscope objectives
- 10x (.40 NA)
- 20x (.75 NA)
- 63x Glycerol (1.30 NA)
- 20x Water (1.00 NA)
- 40x Water (.8 NA)
Leica LAS Lite Software Download