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Leica SP5 Confocal/MultiPhoton with Resonant Scanner - Set to be retired by 2022

What it’s good for

  • Determining colocalization for multiple signals
  • Acquisition of up to 5 color channels simultaneously
  • Acquisition of multiple color channels sequentially
  • Acquisition of a transmitted light image
  • Fixed cells or tissues on a slide with a coverslip (#1.5) or in a dish
  • Live tissues or Live Animals
  • Spectral Deconvolution to correct for overlapping signals
  • Second Harmonic Generation
  • Calcium Dynamics - Fast Scanning with Resonant Scanner
  • FRET
  • Stimulus applications
  • FRAP
  • FLIP
  • Photo-activation
  • Photo-conversion
  • Uncaging

What it’s not good for

  • Live cells in culture: Can be very harsh for some live cell applications and an upright system is not ideal for live cells in a dish. 

Principles of operation

Laser light of specific wavelengths is scanned across the sample and filtered before detection to produce a high resolution image composed of a small optical slice of the sample.

Technical Information

  • Microscope: Leica Upright
  • Incubated 
  • Spectral for all channels
    laser lines
  • 405
  • 458
  • 488
  • 515
  • 543
  • 594
  • 633
  • Mai Tai HP 2-photon laser tunable from 720nm - 1040nm

Microscope objectives

  • 10x (.40 NA)
  • 20x (.75 NA)
  • 63x Glycerol (1.30 NA)
  • 20x Water (1.00 NA)
  • 10x Water (.3 NA)

Leica LAS Lite Software Download

All publications generated utilizing our resources must cite our grant:  NS047101

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