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Leica SP8 Confocal with Lightning Deconvolution

What it’s good for

  • Super Resolution Confocality
  • Determining colocalization for multiple signals
  • Acquisition of up to 4 color channels simultaneously
  • Acquisition of multiple color channels sequentially
  • Acquisition of a transmitted light image with DIC
  • Fixed cells or tissues on a slide with a coverslip (#1.5) or in a dish
  • Spectral Deconvolution to correct for overlapping signals
  • On the fly image deconvolution for super resolution of 120nm in X and Y and 200nm in Z

What it’s not good for

  • Live Cells: This system does not have incubation for live cell applications

Principles of operation

Laser light of specific wavelengths is scanned across the sample and filtered before detection to produce a high resolution image composed of a small optical slice of the sample. Deconvolution further enhances resolution.

Technical Information

  • Microscope: Leica DMi8 Inverted
  • Spectral for all channels
    laser lines
  • 405
  • 488
  • 552
  • 633

Microscope objectives

  • 10x (.40 NA)
  • 20x Oil / Glycerol / Water (.75 NA)
  • 40x Oil (1.30 NA)
  • 60x Oil (1.40 NA)

Leica LAS Lite Software Download