What it’s good for
- Super Resolution Confocality
- Determining colocalization for multiple signals
- Acquisition of up to 4 color channels simultaneously
- Acquisition of multiple color channels sequentially
- Acquisition of a transmitted light image with DIC
- Fixed cells or tissues on a slide with a coverslip (#1.5) or in a dish
- Spectral Deconvolution to correct for overlapping signals
- On the fly image deconvolution for super resolution of 120nm in X and Y and 200nm in Z
What it’s not good for
- Live Cells: This system does not have incubation for live cell applications
Principles of operation
Laser light of specific wavelengths is scanned across the sample and filtered before detection to produce a high resolution image composed of a small optical slice of the sample. Deconvolution further enhances resolution.
Technical Information
- Microscope: Leica DMi8 Inverted
- Spectral for all channels
laser lines - 405
- 488
- 552
- 633
Microscope objectives
- 10x (.40 NA)
- 20x Oil / Glycerol / Water (.75 NA)
- 40x Oil (1.30 NA)
- 60x Oil (1.40 NA)
Leica LAS Lite Software Download