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Hamamatsu Nanozoomer 2.0HT Slide Scanner - Set to be retired by 2022

What it's good for

  • Generating brightfield and multi-color fluorescent images of entire slides or 
    tissue sections automatically
  • 3 fluorescent colors 
  • Fixed cells or sections on a standard 1 x 3 slide with a coverslip (#1.5)
  • Can scan up to 210 slides automatically

What it's NOT good for

  • Thick tissue slices
  • Timelapse

Principles of operation

Fluorescent light from a mercury lamp is filtered for excitation and emission or brighfield light produce an image by scanning specified areas of a slide to generate montage reconstructions.

Technical Information

  • 3 fluorescent color channels and brightfield:
  • DAPI
  • FITC
  • Texas Red
  • Motorized X-Y capability for slide scanning
  • Microscope objective:
  • 20x (.75 NA)

NDP View Software Download

For Slides preparations:

  • No frosted glass
  • No beveled edges of any kind on the slides
  • No label or coverslips hanging off edge or extra mounting medium on edges
  • No wet slides - must be completely dry
  • Use #1 coverslips
All publications generated utilizing our resources must cite our grant:  NS047101