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Gatan 3View / Zeiss Sigma VP Electron Microscope

Available Now!!

This system is capable of performing the following techniques:
  • SEM with high pressure and variable pressure modes
  • TEM on grids with STEM detector and 12 grid specimen holder
  • Serial Block Face Sectioning with Gatan 3View module
  • ATLAS software large scale acquisitions of TEM grids and Array Tomography sections
  • Correlative Microscopy of Light and Electron microscopy techniques
  • Array Tomography with SEM

Principles of operation

Serial block face scanning electron microscopy generates EM resolution 3D images. The system has an ultramicrotome inside the chamber of the SEM, which allows for automatic cutting of a tissue block. The surface of the block is imaged by detection of back-scattered electrons and then a thin section (30-50nm) is cut from the block face. The sample block is then imaged again and a sequence of images can be compiled automatically.

For more information, or access to this technology, please contact Jennifer Santini.


Gatan Serial Block Face Sectioning publications

Serial Block Face Application Note


Probes for Correlative EM:
Colloidal Gold w/wo silver enhancement
DAB Photo-oxidation for EM (i.e., 
Fluorescent Protein, FlAsH and ReAsH, 
Eosin, miniSOG, APEX, mEos4)
Quantum Dots (size and shape)
En bloc autofluorescence/stains (acridine orange)
Fluorescent Proteins (LR White and LR Gold)
LR White section surface immunofluorescence (Array Tomography)

All publications generated utilizing our resources must cite our grant:  NS047101

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