DeltaVision RT Deconvolution Microscope

What it's good for

  • Generating multi-color 3D fluorescent images of high resolution
  • 4 fluorescent colors plus a high contrast black and white DIC channel 
  • Fixed cells or sections on a slide with a coverslip (#1.5)
  • Live cells in a coverslip bottom chamber or dish (#1.5)
  • Cells in culture can be kept at 37 degrees with 5% CO2 
  • Multi-point time-lapse and panel acquisition

What it's NOT good for

  • Thick tissue (>50 microns)  
  • Rapid timelapse
  • Large travel distance

Prnciples of operation

Fluorescent light from a mercury lamp is filtered for excitation and emission. System eliminates out of focus light by calculations AFTER acquisition, producing sharper, brighter images.

Technical Information

  • Microscope Base: Olympus IX70
  • Incubation with CO2 (Solent)
  • 4 fluorescent color channels and DIC:
  • DAPI
  • FITC
  • Texas Red
  • Cy-5
  • Motorized X-Y-Z-T capability for live cell imaging
  • Microscope objectives
  • 10x (.30 NA)
  • 20x (.70 NA)
  • 40x (1.30 NA)
  • 60x (1.40 NA)
  • 100x (1.40 NA)

 

All publications generated utilizing our resources must cite our grant:  NS047101

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