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Michael Castle

Current Research

 

I am an expert in gene therapy specializing in treatment of neurodegenerative disease. Current projects include: 1) intrathecal growth factor gene therapy for Alzheimer's disease, 2) novel vector components that target intravenous gene therapies to disease-affected cells, 3) translational methods for controlling the strength and duration of gene expression in vivo, and 4) new synthetic gene therapies for neurodegenerative disease.

Curriculum Vitae

Citations:

Research Articles

1. Castle MJ, Baltanás FC, Kovacs I, Nagahara AH, Barba D & Tuszynski MH (2020). Postmortem Analysis in a Clinical Trial of AAV2-NGF Gene Therapy for Alzheimer’s Disease Identifies a Need for Improved Vector Delivery. Hum Gene Ther 31 (7-8): 415-22. https://doi.org/10.1089/hum.2019.367 PMID: 32126838.  

2.  Castle MJ, Cheng Y, Asokan A & Tuszynski MH (2018). Physical Positioning Markedly Enhances Brain Transduction After Intrathecal AAV9 Infusion. Sci Adv 4 (11): eaau9859. https://doi.org/10.1126/sciadv.aau9859 PMID: 30443600.

3.  Castle MJ, Gershenson ZT, Giles AR, Holzbaur EL & Wolfe JH (2014). AAV Serotypes 1, 8, and 9 Share Conserved Mechanisms for Anterograde and Retrograde Axonal Transport. Hum Gene Ther 25 (8): 705-29. https://doi.org/10.1089/hum.2013.189 PMID: 24694006.

4.  Castle MJ, Perlson E, Holzbaur EL & Wolfe JH (2014). Long-distance Axonal Transport of AAV9 is Driven by Dynein and Kinesin-2 and is Trafficked in a Highly Motile Rab7-positive Compartment. Mol Ther 22 (3): 554-66. https://doi.org/10.1038/mt.2013.237 PMID: 24100640.

5.  Vandenberghe LH, Bell P, Maguire AM, Cearley CN, Xiao R, Calcedo R, Wang L, Castle MJ, Maguire A, Grant R, Wolfe JH, Wilson JM & Bennett J (2011). Dosage thresholds for AAV2 and AAV8 photoreceptor gene therapy in monkey. Sci Transl Med 3 (88): 88ra54. https://doi.org/101126/scitranslmed.3002103 PMID: 21697530.


Books

1. Castle MJ, editor. Adeno-Associated Virus Vectors: Design and Delivery (2019). Methods in Molecular Biology (New York, NY: Humana Press): 1950. http://doi.org/10.1007/978-1-4939-9139-6


Reviews and Book Chapters

1. Weinholtz CA & Castle MJ (2021). Intersectional Targeting of Defined Neural Circuits by AAV Vectors. J Neurosci Res 99 (4): 981-90. https://doi.org/10.1002/jnr.24774 PMID: 33341969

2. Hunter JE, Gurda BL, Yoon SY, Castle MJ & Wolfe JH (2019). In Situ Hybridization for Detection of AAV-Mediated Gene Expression. In: Adeno-Associated Virus Vectors: Design and Delivery (Castle MJ, ed). Meth Mol Biol (New York, NY: Humana Press) 1950: 107-22. https://doi.org/10.1007/978-1-4939-9139-6_6  PMID: 30783970                

2.  Castle MJ, Turunen HT, Vandenberghe LH & Wolfe JH (2016). Controlling AAV tropism in the nervous system with natural and engineered capsids. In: Gene Therapy for Neurological Disorders (Manfredsson FP, ed). Meth Mol Biol (New York, NY: Humana Press) 1382: 133-49. https://doi.org/10.1007/978-1-4939-3271-9_10 PMID: 26611584

Postdoctoral Fellow
Michael Castle, Ph.D.
Assistant Project Scientist