JCI Core Service Request Form
The goal of the Joint Cell Isolation (JCI) Core is to provide MARC members with services for the isolation of tissue fragments or single cells from human or animal joint specimens. The Core provides
laser capture microdissection
flow cytometry cell sorting
Laser Capture Microdissection (LCM)
The JCI Core provides staff-operated laser capture microdissection of tissue regions or cells from sections of human or animal joints. As seen below, the LCM is a microscope that is capable of dissecting tissue on a semipermeable microscope slide. An image of the slide is projected onto a computer where the desired tissue/cell is outlined and
ultraviolet or infrared solid-state lasers cut out the tissue effectively and accurately. We also have a CryoStar NX70 cryostat and a
Leica RM2125 RTS microtome to section the tissue for slide preparation prior to using the LCM. A
demo provided by ThermoFisher illustrates how the LCM can dissect out tissue/structures/cells from a tissue sample.
Services offered for the LCM
- Tissue sectioning
- Slide staining
- Laser capture microdissection
- Ultraviolet laser
- Infrared laser
- Post-capture nucleic acid extraction
See our services
page for more information on services provided or to request a service or consultation.
Flow Cytometry Cell Sorting
The JCI Core provides cell sorting services through integration with the state of the art La Jolla Institute Flow Sorting Facility.
- Staff-operated cell sorting
- User-operated cell sorting
- Joint tissue disaggregation