Non-invasive Vaccination, Mass Spectrometry, Proteomics, Secretome, Ultrafiltration
Dr. Huang received his PhD in National Taiwan University. Following four years as Assistant professor of Dermatology at University of Alabama at Birmingham, he was recruited as Associate Professor to La Jolla Institute for Molecular Medicine, San Diego. Dr. Huang then joined UCSD Dermatology in 2006. His research is primarily supported by the NIH. Dr. Huang and his colleagues have recently developed a novel technique using various capillary ultrafiltration (CUF) probes in conjunction with mass spectrometric proteomics. The CUF probes allow them to capture tumor secretions in vivo. This technique will also make a major impact on systems biology studies of tumor-host cell interactions.
Traditionally, the administration of a vaccine usually requires one or more needle injections performed by trained medical personnel. The concept of a needle-free noninvasive technology may reduce medical costs by allowing personnel with limited medical training to administer the vaccine. Furthermore, the use of noninvasive routes for vaccine delivery such as the bare skin or the nasal cavity may be advantageous for vaccination since there is a large amount of associated lymphoid tissue and antigen presenting cells (APCs) near the skin surface. Huang's laboratory has been demonstrating that animals can be effectively immunized by epicutaneous or intranasal applications of either adenovirus- or E. coli-vectored vaccines.
The noninvasive vaccines they have been generating include anti-tumor, anthrax, and P. acnes vaccines. Two future directions in this laboratory will be (1) to understand the mechanism of noninvasive vaccines, and (2) to improve the efficiency and safety of noninvasive vaccines.