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Microscope: NikonUSA
Stanford University Cell Sciences Imaging Facility
(wealth of information)
Secondary antibodies:
Molecular Probes Inc.
(good inventory of alexafluor antibodies)
Jackson ImmunoResearch
(great inventory of secondaries: cat, rat, horse…)
Note on secondary antibodies: To reduce the possibility of artifactual cross-reaction of a secondary antibody to inappropriate primary antibodies in multi-plexed staining, use secondary antibodies that have been affinity-purified to limit cross-reaction.
Protocols for staining and troubleshooting:
Dako Cytomation
(great staining and troubleshooting tips)
BD Biosciences