Microscopy Links

Microscope: NikonUSA
Stanford University Cell Sciences Imaging Facility (wealth of information)
Secondary antibodies:
- Molecular Probes Inc. (good inventory of alexafluor antibodies)
- Jackson ImmunoResearch (great inventory of secondaries: cat, rat, horse…)
- Note on secondary antibodies: To reduce the possibility of artifactual cross-reaction of a secondary antibody to inappropriate primary antibodies in multi-plexed staining, use secondary antibodies that have been affinity-purified to limit cross-reaction.
Protocols for staining and troubleshooting:
- Dako Cytomation (great staining and troubleshooting tips)
- BD Biosciences