Director
Thomas J. Kipps, MD, PhD
Professor of Medicine
Phone: 858-534-5400
E-mail:
tkipps@ucsd.edu
Contact
Dennis Young
Phone: 858-822-0407
Fax: 858-822-0403
E-mail:
djyoung@ucsd.edu
Location
UC San Diego Moores Cancer Center
3855 Health Sciences Drive, MC 0803
La Jolla, CA 92093-0803
Room 2317-Q
Service Profile
- Aseptic sorting of living cell populations (up to 4 simultaneous collection tubes)
- Direct cloning into microtiter tissue culture plates or onto microscope slides
- Isolation of rare cell subpopulations or large number of cells
- Quantitative fluorescence analysis (up to 9 colors)
- Consulting (design of experiments; analysis or conversion of data)
- Typical applications include:
- Viability and toxicity assays
- Apoptosis assays (TUNEL, sub-G1, caspases, Annexin V, mitochondrial membrane potential, etc)
- Calcium Flux
- Intracellular pH
- Degranulation
- Oxidative Burst
- Phagocytosis
- Reticulocytes
- Platelets
- Bead-based assays
- For more information, see
Service Details
Equipment Profile
- BD FACSAria (High speed, 9-color, triple-laser cell sorter)
- Automated Cell Deposition Unit (ACDU)
- 4-way sorting
- BD FACSAria II SORP (High speed, 13-color, 4-laser cell Sorter)
- Automated Cell Deposition Unit (ACDU)
- 4-way sorting
- BIO-RAD S3 (2-laser, 4-color cell sorter)
- BD FACSCalibur (2) - 4-color, non-sorting cytometers
Overview
The speed at which the automated instruments of this resource can examine or separate free floating cell populations for several parameters - sometimes simultaneously - and chart the results, provides powerful opportunities for obtaining data on cell populations of statistically reliable size.
More information