COVID-19 Updates

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Flow Cytometry Core Facility


Flow cytometry core facility has reopened.

Core Manager (primary contacts): Myungja Ro

The Flow Cytometry Core facility is now open at UC San Diego Altman Clinical and Translational Research Institute (ACTRI). This facility provides a high-end flow cytometer to identify, analyze and quantify cells in several sample types. It is equipped with a ZE5 Cell Analyzer from Bio-Rad, which is a benchtop system used for analysis (not sorting). The ZE5 offers great flexibility in sample handling with the ability to use several sample formats, such as single tubes, tube racks (up to 40 tubes/rack), 96-well plates (U- and V-bottom) and 384-well plates. The built-in sample loader on the ZE5 minimizes hands-on time for sample loading, allows for sample mixing with adjustable orbital vortex shaker and provides active temperature regulation of samples. The ZE5 also has a new and innovative software allowing for easy experiment design and set-up.

The ZE5 in the ACTRI flow core has four lasers (violet (407nm), blue (488nm), yellow-green (561nm) and red (640nm), enabling detection of up to 15 fluorometric parameters, including SSC. The ZE5 is equipped with a ZE5-Eye monitor to ensure that researchers have the right filters in place for their experiments, avoiding errors associated with misplaced filters and loss of detection.

ZE5-Configuration

Location:
Altman Clinical and Translational Research Institute (ACTRI)
University of California San Diego
9452 Medical Center Dr.
Suite L2 E317
La Jolla, CA 92037

Hours:
9 a.m. to 3 p.m., daily

Presently, the facility is only offering self-operated use. Prior to self-operating the equipment, researchers must complete a two-hour training session (at least 2 hours, $200/ hour). Assisted use may be available under certain circumstances and after discussion with the core manager.

For more information or to schedule appointments for initial training and access to the ZE5, please contact the flow cytometry core manager.

Core Manager (primary contacts):  Myungja Ro

Parking:
If you are visiting our facility from another part of the UC San Diego campus or are a non-UC user, we will reimburse your parking by subtracting from the recharge time you spend using our core. You must provide a parking receipt for your core time use.

Recharge Rates


 
  UC UsersNon-UC Users
Self-operated use only
Training
$40/hour
$200/hour
$150/hour
$300/hour
Manager consultation
$60/hour$90/hour

Data

Files in FSC folders (folders for saving data) in the flow core computer are deleted every first day in January, April, July and October. Please make your own copy of your data before the day (March 31, June 30, September 30 and December 31). Deleted data cannot be recovered. 
 

Rules for COVID-19 - Please consult with core manager about COVID precautions before using the flow again

1.     Social Distancing Occupancy Limit: 1
2.     You are required to wear a face covering at all times.
3.     Gloves need to be worn at all times.
4.     Disinfect desk and work area with 70% alcohol.