Biomarker Technology Resources (BTR)

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The CTRI Biomarker Technology Resource (BTR) is a family of laboratories with expertise in advanced techniques and/or indication-specific expertise that the CTRI has developed collaborative relationships with. The Individual BTRs work with the Biorepository and Biomarker Lab to provide seamless biomarker determination, from acquisition to analysis.

Proteomics

The proteomics core is a mass spectrometry based core that has expertise in a i) protein sequencing, ii) post translational modification (PTM) characterization and, iii) relative and absolute protein quantifications. The core provides analyses on state-of-the-art mass spectrometers for the highest quality data. The core can identify protein antigen targets in pathogens and transplant tissue and determine the constituents of protein complexes. The core has years of experience in PTM characterizations such as protein Ser/Thr and Tyr phosphorylation site determination, protein ubiquitination, protein methylaion and acetylation, etc. Protein quantification is the method of choice for disease biomarker discovery in serum and tissue samples. The core has expertise in both label-free and labeled protein quantification approaches and can help you customize these approaches to your specific experimental designs.  

Orthopedics

The Muscle Physiology Laboratory is affiliated with both the Orthopaedic Surgery Department in the School of Medicine and the Bioengineering Department within the School of Engineering. As such, a variety of methodologies are available to define both the structure and function of biological tissues. These methods include, micro computed tomography, simultaneous high resolution microscopy and mechanics of single muscle cells and small muscle fiber bundles, metabolic assays for insulin sensitivity and glucose transport as well as the traditional methodologies.

Our laboratory also has a significant commitment to training students via scientific research. Training occurs at all levels ranging from high school to post-graduate international visiting fellows. Lab personnel specialize in studying musculoskeletal design and musculoskeletal adaptation. UCSD faculty as well as scholars from other universities regularly partner with the lab’s physiologists, hand surgeons, residents, graduate students, and undergraduate students. Please visit the lab’s webpage at http://muscle.ucsd.edu/ for more information about current projects, funding recent publications, and opportunities for collaboration.

Human Tissue Resource

The Human Tissue Repository (Biorepository) specializes in the collection and storage of human tissues following surgical resections. The collections are consented by the participants and the consents are approved by the Institutional Review Board (IRB) prior to the collection of samples.  The tissues are handled by Pathologists, Certified Pathologists’ Assistant and Research Associates and the tissues are stored according to the standard protocols to maintain the quality of the tissues with basic information and medical records.  The data management is maintained by a highly secured bioinformatics with easy computerized tracking and linking of tissues and medical information, protected by HIPAA and ethical requirements.  The Human Tissue Resource offers clinical and translational services; tissue-based research services; tissue preservation techniques; resource management; and a wide variety of Anatomic Pathology laboratory services including tissue processing, histology (H and E), and Immunohistochemistry as well as Laser Capture-Microdissection (LCM) that captures and pools specific cell population within a tissue specimen thus facilitating the analysis of a heterogeneous tissue to accurately assess a specific analyte within cell populations of interest.  The Human Tissue Resource also provides centralized support structure for existing sample repositories and it is open to other research institutions or investigators that may necessitate the storing and processing of their Biospecimens.

For inquiries and availability, please send an email to ctrilab@ucsd.edu