The GlycoAnalytics Core is a fully equipped laboratory with specialized equipment for glycan analysis.
Thermo Scientific Dionex Ion Chromatography (IC) System HPAEC-PAD/UV/FL Detector
Equipped with an electrochemical, UV and fluorescence detector; dual gradient pump and AS 50 autosampler. It is used for the composition analysis of monosaccharides, oligo- and polysaccharide analysis. The instrument is also capable of analysis of Sulfate and Phosphate using the IonPac column and conductivity detector. Oligosaccharides in limited quantity can also be purified using an online de-salter for collection of appropriate fractions.
Agilent Technologies 7820A GC System 5975 Series Gas Mass Selective Detector (GC-MS)
The GC-MS system is equipped with Agilent Technologies 7820A gas chromatograph system with Rtx-5ms capillary column (30m x 0.25mm x 0.25µm) and coupled to 5975 mass selective detector. This instrument can detect pM amount of samples and is primarily used for monosaccharide and linkage analysis of glycans. This instrument is also used for fatty acid and amino acid analysis upon request from clients.
Applied Biosystems QSTAR (MALDI-qTOF/ESI hybrid MS/MS)
The instrument is a hybrid quadrupole time-of-flight mass spectrometer, this configuration is ideally suited for the analysis of all classes of glycoconjugates including free glycans, glycopeptides, and glycolipids. This instrument is specifically used for N-linked and O-linked oligosaccharides in both positive and negative mode. The tandem mass fragmentation is done using Argon as collision gas.
Thermo Scientific LTQ-XL Orbitrap Discovery Electrospray Ionization Mass Spectrometer
An LTQ Orbitrap Discovery electrospray ionization mass spectrometer (Thermo Scientific) equipped with Thermo Scientific Ultimate 3000 HPLC system. This system also has an online Ultimate 3000 variable wavelength detector. This system is primarily used for analysis of sialic acids, neutral and charged glycans and disaccharides generated from glycosaminoglycan.
The instrument is an Ultra pressure liquid chromatography equipped with fluorescent detector and high resolution column. This system is used for analysis of DMB tagged sialic acid and 2-AB tagged N-linked glycans in low pico-mole to femto-mole amounts.
sciFLEXARRAYER S3 Micro-Array Printer
SciFLEXARRAYER S3 is a contactless spotter with automated ultra-low volume liquid handling of various types of samples, including biological materials or cells. This instrument provides high-speed printing through a ceramic piezo element fitted around glass capillaries for generating spots ranging from 50 - 1000 μm, which can be arrayed with < 3 μm precision and < 5μm accuracy. The instrument is equipped with a live-stream camera and array quality control software to ensure high fidelity array production. The S3 system offers optimal balance of performance and throughput and is ideal for application development as well as routine printing of glycan, protein and DNA arrays.
The GlycoAnalytics Core also has access to an NMR and provides NMR analysis of glycans.