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Takaki Komiyama, PhD

Takaki Komiyama, PhD

Professor, Neurobiology Section and Neurosciences Department

Contact Information

Phone: 858.534.4654

Location: CNCB 304

Mailing Address:
University of California, San Diego
9500 Gilman Drive #0634
La Jolla, CA 92093

Lab Website

Research Title
Cortical microcircuits and behavior

Research Description
Animals constantly modify their behavior through experience. Flexible behavior is key to our ability to adapt to the ever-changing environment. Our laboratory is interested in studying the activity of neuronal ensembles in behaving animals, and how it might change with experience and learning. The main technique we use is in vivo two-photon calcium imaging. This technique enables the recording of the activity of large neuronal ensembles in intact animals. Combining this with genetically-encoded calcium indicators, we can image the same neuronal populations over days, weeks and months. The use of transgenic mouse lines allows the identification of molecularly-defined neuron types.

We are among the pioneers to use this imaging technique in awake, behaving mice that are head-fixed under two-photon microscopes. We are developing a number of behavioral paradigms for head-fixed mice to address fundamental questions in systems neuroscience; how are sensory representations shaped by brain state and sensory experience? How does the brain learn new motor skills? How does the brain support flexible behavior? We are tackling these questions, combining a variety of techniques including in vivo two-photon imaging, behavioral, genetic, electrophysiological and pharmacogenetic techniques.

  1. Mitani, A. and Komiyama, T. (2018) Real-Time Processing of Two-Photon Calcium Imaging Data Including Lateral Motion Artifact Correction. Frontiers in Neuroinformatics, DOI:10.3389/fninf.2018.00098
  2. Lu, Y., Liu, X., Hattori, R., Ren, C., Komiyama, T., and Kuzum, D. (2018) Ultra-low Impedance Graphene Microelectrodes with High Optical Transparency for Simultaneous Deep 2-photon Imaging in Transgenic Mice. Advanced Functional Materials, DOI:10.1002/adfm.201800002.
  3. Li, W.L., Chu, M., Wu, A., Suzuki, Y., Imayoshi, I., and Komiyama, T. (2018) Adult-born neurons facilitate olfactory bulb pattern separation during task engagement. eLife, 2018;7:e33006.
  4. Mitani, A., Dong, M. and Komiyama, T. (2018) Brain-computer interface with inhibitory neurons reveals subtype-specific strategies. Current Biology, 28(1), 77-83.
  5. Chu, M.W., Li, W.L. and Komiyama, T. (2017) “Lack of pattern separation in sensory inputs to the olfactory bulb during perceptual learning.” eNeuro, 0287-17.201.
  6. Hwang, E.J.*, Dahlen, J.E.*, Mukundan, M. and Komiyama, T. (2017) “History-dependent action selection bias in the posterior parietal cortex.” Nature Communications, 8, 1242.
  7. Hattori, R.*, Kuchibhotla, K.*, Froemke, R.#, and Komiyama, T.#(2017) Functions and dysfunctions of neocortical inhibitory neuron subtypes. (Review) Nature Neuroscience, 20, 1199-1208.
  8. Peters, A.J., Lee, J., Hedrick, N.G., O'Neil, K., and *Komiyama, T*. (2017) Reorganization of corticospinal output during motor learning. *Nature Neuroscience*, 20, 1133-41 - *Rated by Faculty of 1000*
  9. Makino, H.*#, Ren, C.*, Liu, H.*, Kim, A.N., Kondapaneni, N., Liu, X., Kuzum, D. and Komiyama, T.#(2017) Transformation of cortex-wide emergent properties during motor learning. Neuron, 94(4), 880-90. Preview by Banerjee and Long, Neuron 94(4), 698-700.
  10. Peters, A.J., Liu, H. and *Komiyama, T.* (2017) Learning in the rodent motor cortex. (Review) *Annual Review of Neuroscience*, 40, 77-97.
  11. McIntyre, J.C., Thiebaud, N., McGann, J.P., Komiyama, T. and Rothermel, M. (2017)
    Neuromodulation in chemosensory systems. (Review) Chemical Senses, 42(5), 375-9.
  12. Makino, H.*, Hwang, E.J.*, Hedrick, N.G.* and Komiyama, T.
    “Circuit mechanisms of sensorimotor learning.” (Review)
    Neuron, 92(4), 705-21.
  13. Chu, M.W., Li, W.L. and Komiyama, T. (2016)
    “Balancing the robustness and efficiency of odor representations during learning.”
    Neuron, 92(1), 174-86.
  14. Makino, H. and Komiyama, T. (2015)
    “Learning enhances the relative impact of top-down processing in the visual cortex.”
    Nature Neuroscience, 18(8), 1116-22. PMID: 26167904
  15. Chen, S.X., Kim, A.N., Peters, A.J. and Komiyama, T.(2015)
    “Subtype-specific plasticity of inhibitory circuits during motor learning.”
    Nature Neuroscience, 18(8), 1109-15. PMID: 26098758
    a. News and Views by Grillo, F.W., West, L. and De Paola, V., Nature Neuroscience 18(8), 1062-64.
  16. Boyd, A., Kato, H.K., Komiyama, T. and Isaacson, J.S. (2015)
    "Broadcasting of cortical activity to the olfactory bulb."
    Cell Reports, 10(7), 1032-9. PMID: 25704808
    a. Rated by Faculty of 1000
  17. Peters, A.J., Chen, S.X. and Komiyama, T. (2014)
    “Emergence of reproducible spatiotemporal activity during motor learning.”
    Nature, 510(7504), 263-7. PMID: 2480523
    a. Rated by Faculty of 1000
  18. Guo, Z., Hires, S.A., Li, N., O’Connor, D., Komiyama, T., Ophir, E., Huber, D., Bonardi, C., Morandell, K., Gutnisky, D., Peron, S., Xu, N., Cox, J. and Svoboda, K. (2014)
    “Procedures for behavioral experiments in head-fixed mice.“
    Plos ONE, 9(2) PMID: 24520413
  19. Kato, H.K., Gillet, S.N., Peters, A.J., Isaacson, J.S.# and Komiyama, T.# (2013)
    “Parvalbumin-expressing interneurons underlie a local inhibitory circuit regulating the gain of ilfactory bulb output.”
    Neuron, 80, 1218-31. PMID: 24239124
    a. Preview by Uchida, N., Eshel, N. and Watabe-Uchida, M., Neuron 80, 1106-09.
    b. ‘Research Highlight’ by Yates, D., Nature Reviews Neuroscience 15, 4-5.
    c. Rated by Faculty of 1000
  20. Kato, H.K.*, Chu, M.W.*, Isaacson, J.S. # and Komiyama, T. # (2012)
    “Dynamic sensory representations in the olfactory bulb: modulation by wakefulness and experience.”
    Neuron, 76(5), 962-75. PMID: 23217744
    a. Selected for the video abstract of the issue
    b. Rated by Faculty of 1000
  21. Sweeney, L.B., Chou, Y-H., Wu, Z., Joo, W., Komiyama, T., Potter, C., Kolodkin, A., Garcia, K.C. and Luo, L. (2011)
    “Secreted Semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting.”
    Neuron, 72, 734-47. PMID: 22153371
  22. Komiyama, T.*, Sato, T.R., O’Connor, D.H., Zhang, Y.X., Huber, D., Hooks, B.M., Gabbito, M. and Svoboda, K. (2010)
    “Learning-related fine-scale specificity imaged in motor cortex circuits of behaving mice.” Nature, 464(7292), 1182-6. PMID: 20376005 (*: correspondence)
    a. Rated by Faculty of 1000
  23. O’Connor, D.H., Clack, N.G., Huber, D., Komiyama, T., Myers, E.W. and Svoboda, K. (2010)
    “Vibrissa-based object localization in head-fixed mice.”
    J. Neuroscience, 30, 1947-67. PMID: 20130203