Understanding how experience controls brain development
My research is focused on understanding the mechanisms by which experience controls the development of the brain. My lab addresses fundamental questions about circuit development, synaptogenesis and plasticity by examining the development of the visual system in Xenopus tadpoles, using in vivo time lapse imaging, combined with both gene transfer and electrophysiological studies of visual system function. We have determined many of the genetic components and cellular mechanisms that are required for the development of brain circuits. These studies provide fundamental information regarding experience-dependent mechanisms of brain development and may provide insight into developmental brain disorders.
Thirumalai, V., and Cline, H.T. (2008). Endogenous dopamine suppresses initiation of swimming in pre-feeding zebrafish larvae. J Neurophysiol. 100: 1635-1648 PMCID: PMC2544474
Van Keuren-Jensen, K.R., and Cline, H.T. (2008). Homer proteins shape Xenopus optic tectal cell dendritic arbor development in vivo. Dev Neurobiol. 68: 1315-1324. PMID: 18636533
Cantallops, I., and Cline, H.T. (2008). Rapid activity-dependent delivery of the neurotrophic protein CPG15 to the axon surface of neurons in intact Xenopus tadpoles. Dev Neurobiol 68, 744-759. PMID: 18383547
Chiu, S.L., Chen, C.M., and Cline, H.T. (2008). Insulin receptor signaling regulates synapse number, dendritic plasticity, and circuit function in vivo. Neuron 58, 708-719. PMID: 18549783
Bestman, J.E., and Cline, H.T., (2008) The RNA binding CPEB controls dendrite morphogenesis and neural circuit assembly in vivo. PNAS: 105: 20494-20499 PMCID: PMC2629308
Ewald, R.C., and Cline, H.T., (2009) Cloning and characterization of NMDA receptor subunits in Xenopus laevis tadpoles. Front. Mol. Neurosci. (In Press) PMC Journal – In Process
Chen, C-M, Chiu, S-L, and Cline, H.T., (2009) Co-expression of Argonaute2 enhances short hairpin RNA-induced RNA interference in Xenopus CNS neurons in vivo. Front. Neuromethods (In Press) PMC Journal - In Process
Shen, W., Santos Da Silva, J., and Cline, H.T. (2009) GABAAR-dependent synaptic transmission sculpts dendritic structure in vivo. J Neurosci. 2009 Apr 15;29(15):5032-43.